质粒名称:pC-HYG-YR
质粒编号:61765
质粒备注:
Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).
载体名称:pCAMBIA-0380
载体大小:6812
完整质粒大小:10737
载体类型:Ternary vector for Agrobacterium mediated transformation of fungi
筛选标记:Hygromycin, URA3
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Hygromycin
物种来源:Synthetic
启动子:trpC promoter
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:HindIII (not destroyed)
5'测序引物:LB-F1 (5'-gtggtgtaaacaaattgacgc-3')
3'测序引物:RB-F1 (5'-ggataaaccttttcacgccc-3')
质粒图谱: