质粒名称：pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)
质粒编号：107942
质粒备注：

Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

载体名称：pCAG-CFP
载体类型：哺乳动物表达

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
基因别名：AAS1815
基因别名：enhanced, high-fidelity version of Cas12a from Acidaminococcus sp. BV3L6
物种来源：Acidaminococcus sp. BV3L6
基因突变：E174R, S542R, K548R, and N282A
启动子：CAG
标签/融合蛋白：· NLS (nucleoplasmin) (C terminal on insert) · 3x HA (C terminal on insert)

克隆方法：Gibson Cloning
5'测序引物：pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
3'测序引物：Bglob-pA-R (5'-ttttggcagagggaaaaaga-3')

质粒图谱：