质粒名称:PB_tre_Cas9
质粒编号:126029
质粒备注:
PiggyBac cargo vector expressing doxycycline inducible Cas9
载体名称:piggyBac cargo vector
完整质粒大小:11497
载体类型:Mammalian Expression, CRISPR ; piggyBac
筛选标记:潮霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:humanized S. pyrogenes Cas9
插入大小:4272
启动子:TRE
克隆方法:Gibson Cloning
5'测序引物:GGGAAAAGGCCTCCACGG
质粒图谱: