质粒名称:Inducible Caspex expression
质粒编号:97421
质粒备注:
For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
载体名称:PB-TetON
载体大小:7501
载体类型:哺乳动物表达,CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:Caspex
基因别名:CATG
基因别名:Genomic Locus Proteomics
物种来源:S. pyogenes, Soybean
基因突变:dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, A134P
启动子:TRE3G
标签/融合蛋白:· 3xFLAG (N terminal on insert) · V5 (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:ggcctccacggccactagt
3'测序引物:EF1a-rev
质粒图谱: