质粒名称:CD63-pEGFP C2
质粒编号:62964
质粒备注:
full length human CD63 cloned into pEGFP C2
载体名称:pEGFP C2
载体大小:4700
载体类型:哺乳动物表达
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:CD63 molecule
物种来源:H. sapiens (human)
基因突变:CD63 has 1 silent mutation compared to the coding sequence of NM_001267698.1 N129 AAT instead of AAC
插入大小:749
基因名称:CD63 (a.k.a. AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30)
启动子:CMV
克隆方法:限制性内切酶
5'克隆位点:KpnI (not destroyed)
3'克隆位点:BamHI (not destroyed)
5'测序引物:5’ CCGACAACCACTACCTGAGC 3’
3'测序引物:5’ ACAAACCACAACTAGAATGCAG 3’
质粒图谱: