质粒名称:pKCcas9dO
质粒编号:62552
质粒备注:
High efficiency Streptomyces genome editing by CRISPR/Cas9 system
载体名称:pKC1139
载体类型:Bacterial Expression, CRISPR ; Bacterial Genome Editing
原核抗性:Apramycin, 25 μg/mL
培养温度:30℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:Scocas9
物种来源:artificially synthesized, codon optimized gene from Strep pyogenes
插入大小:4107
启动子:ptipA
克隆方法:限制性内切酶
5'克隆位点:NdeI (not destroyed)
3'克隆位点:EcoRI (unknown if destroyed)
5'测序引物:unknown
3'测序引物:M13pUC-Rev
质粒图谱: