质粒名称:pFHMSP-LIC-N
质粒编号:26095
质粒备注:
(Empty Backbone)Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
载体名称:pFHMSP-LIC-N
载体类型:Insect Expression ; Baculovirus expression
原核抗性:Ampicillin, 100 渭g/mL
培养温度:37掳C
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
启动子:polyhedrin
标签/融合蛋白:· His (N terminal on backbone) · TEV cleavage site (N terminal on backbone)
克隆方法:Ligation Independent Cloning
5'测序引物:pFHMSP-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
3'测序引物:pFHMSP-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')