质粒名称:pFA6a-GFP(S65T)-His3MX6
质粒编号:41598
载体名称:pFA6a-kanMX6
载体大小:3938
载体类型:Yeast Expression ; Yeast genomic targeting
筛选标记:HIS3
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:His3
基因别名:k-HIS3
基因别名:SKHIS3
物种来源:S. kluyveri
插入大小:2348
启动子:TEF
标签/融合蛋白:· GFP (S65T) (N terminal on insert) · yeast ADH1 terminator (N terminal on insert) · A. gossypii translation elongation factor 1a gene promoter (N terminal on insert) · A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:PacI (not destroyed)
3'克隆位点:PmeI (not destroyed)
5'测序引物:SP6
3'测序引物:T7
质粒图谱: