质粒名称:pDD162 (Peft-3::Cas9 + Empty sgRNA)
质粒编号:47549
质粒备注:
Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
载体名称:pCFJ601
载体大小:2300
完整质粒大小:8113
载体类型:Worm Expression, CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Mach1
高拷贝/低拷贝:高拷贝
插入基因:Cas9
物种来源:Synthetic
基因突变:Codon optimized and with synthetic introns for C. elegans
插入大小:4323
启动子:eef-1A.1 (eft-3)
标签/融合蛋白:· NLS (C terminal on insert) · HA (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:TCAGTTGGGAAACACTTTGCT
3'测序引物:gcttgaaaggattttgcatttatc
质粒图谱: