质粒名称:sgRNA(MS2) cloning backbone
质粒编号:61424
质粒备注:
(Empty Backbone)sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
载体名称:pUC19
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
启动子:U6
克隆方法:限制性内切酶
5'测序引物:CACCTCTGACTTGAGCGTCGATTTTTGTG
3'测序引物:CCCGCCGCGCTTAATGC
质粒图谱: