质粒名称:pSH-EFIRES-B-AtAFB2-mCherry
质粒编号:129718
质粒备注:
AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry
载体名称:pSH-EFIRES-B
载体大小:8000
完整质粒大小:10442
载体类型:Mammalian Expression, CRISPR, TALEN ; Human safe harbor locus (A AVS1) site-specific integration
筛选标记:Blasticidin ; mCherry for FACS enrichment
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:AFB2
基因别名:auxin signaling F-box 2
物种来源:A. thaliana (mustard weed)
基因突变:codon optimized
插入大小:2445
基因名称:AFB2 (a.k.a. AT3G26810, auxin signaling F-box 2)
启动子:EF1a
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:NotI (not destroyed)
5'测序引物:tctctccacaggtgtccact
3'测序引物:acaccggccttattccaagc
质粒图谱: