质粒名称:pSaCas9_GFP
质粒编号:64709
质粒备注:
Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
载体名称:pCAG
载体大小:4345
完整质粒大小:8398
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH10B
高拷贝/低拷贝:高拷贝
插入基因:SaCas9-2A-GFP
基因别名:Staphylococcus aureus Cas9
物种来源:Synthetic
插入大小:4053
启动子:CAG
标签/融合蛋白:· 3xFLAG (N terminal on insert) · SV40 NLS (N terminal on insert) · 2A-GFP (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:PstI (not destroyed)
5'测序引物:ggctctagtgcctctgctaacc
3'测序引物:M13R
质粒图谱: