质粒名称:pRGEB32
质粒编号:63142
质粒备注:
(Empty Backbone)Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
载体名称:pRGEB31
载体类型:Plant Expression, CRISPR
筛选标记:潮霉素
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
启动子:Rice snoRNA U3 promoter for gRNA/PTG expression and UBI promoter for Cas9 expression
克隆方法:限制性内切酶
5'测序引物:M13R(-48)
3'测序引物:M13F(-20)
质粒图谱: