质粒名称:pPRIME-TET-GFP-FF3
质粒编号:11662
质粒备注:
3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
载体名称:pPRIME-TET-GFP
载体大小:8226
载体类型:哺乳动物表达,慢病毒
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:FF3
基因别名:firefly luciferase hairpin
物种来源:Firefly
插入大小:120
克隆方法:限制性内切酶
5'克隆位点:XhoI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:EGFP-C
质粒图谱: