质粒名称:pMonAID_dCas9-PmCDA_Hyg_ALS
质粒编号:91692
质粒备注:
Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls
载体名称:pZDgRNA_Cas9ver.2 (D10A)_HPT
载体大小:17773
载体类型:Plant Expression
筛选标记:潮霉素
原核抗性:Spectinomycin, 50 μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:SpCas9
物种来源:Streptococcus pyogenes
基因突变:D10A and H840A for dead Cas9
启动子:2x35S
克隆方法:Gibson Cloning
5'测序引物:ggaagttcatttcatttggagag
3'测序引物:ccatttgcattttgatgtccg
质粒图谱: