质粒名称:pLOX-TERT-iresTK
质粒编号:12245
质粒备注:
2nd generation lentiviral vector for reversible immortalization of human primary cells
载体名称:pHR'
载体大小:13115
载体类型:Mammalian Expression, Lentiviral, Cre/Lox
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:TERT, HSV1-TK
基因别名:TERT
基因别名:HSV-1 thymidine kinase
物种来源:H. sapiens (human)
基因突变:LoxP in 3' LTR.
基因名称:TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
克隆方法:限制性内切酶
5'测序引物:CMV Fwd for hTert
3'测序引物:SP6 primer rev for LoxP
质粒图谱: