质粒名称:pLL3.7
质粒编号:11795
质粒备注:
(Empty Backbone)3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
载体名称:pLL3.7
载体大小:7650
载体类型:Mammalian Expression, Lentiviral, RNAi, Cre/Lox
筛选标记:GFP
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:None
启动子:mouse U6
克隆方法:限制性内切酶
5'克隆位点:HpaI (not destroyed)
3'克隆位点:XhoI (not destroyed)
5'测序引物:U6 (5'-cagtgcaggggaaagaatagtagac-3')
质粒图谱: