质粒名称:pLKO.1 puro
质粒编号:8453
质粒备注:
(Empty Backbone)3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
载体名称:pLKO.1
载体大小:7032
载体类型:Mammalian Expression, Lentiviral, RNAi
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:None
基因别名:LKO.1
克隆方法:限制性内切酶
5'克隆位点:AgeI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:LKO.1 5'
质粒图谱: