质粒名称:pJMP1
质粒编号:79873
质粒备注:
Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA
载体名称:pAX01
载体大小:7781
完整质粒大小:12080
载体类型:Bacterial Expression, CRISPR
筛选标记:Bacillus subtilis erythromycin/lincomycin marker
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:dCas9
基因别名:catalytically inactive Cas9
物种来源:Streptococcus pyogenes
基因突变:D10A, H840A
插入大小:4107
启动子:xylA
克隆方法:限制性内切酶
5'克隆位点:BamHI (not destroyed)
3'克隆位点:BamHI (not destroyed)
5'测序引物:TCCTTTGTTTATCCACCGAAC
3'测序引物:GCCTCGTGATACGCCTATTT
质粒图谱: