质粒名称:pJA50
质粒编号:59931
质粒备注:
gRNA for cleavage at unc-58(e665) locus in C elegans
载体名称:pRB1017
载体类型:CRISPR
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:高拷贝
插入基因:unc-58(e665) gRNA
物种来源:C. elegans (nematode)
插入大小:19
启动子:U6
克隆方法:限制性内切酶
5'克隆位点:BsaI (destroyed during cloning)
3'克隆位点:BsaI (destroyed during cloning)
5'测序引物:M13 For
3'测序引物:M13 Rev
质粒图谱: