质粒名称:pICH86966::AtU6p::sgRNA_PDS
质粒编号:46966
质粒备注:
Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter
载体名称:pICH86966
载体大小:6340
完整质粒大小:6552
载体类型:CRISPR ; Plant expression
筛选标记:Kanamycin
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:AtU6p::sgRNA_PDS
物种来源:Synthetic
插入大小:212
克隆方法:限制性内切酶
5'克隆位点:Bsa I (destroyed during cloning)
3'克隆位点:Bsa I (destroyed during cloning)
5'测序引物:gtggtgtaaacaaattgacgc
3'测序引物:ggataaaccttttcacgccc
质粒图谱: