质粒名称：pHLmMBP-10
质粒编号：72348
质粒备注：

(Empty Backbone)Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

载体名称：pHLmMBP-10
载体大小：5931
载体类型：哺乳动物表达

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

启动子：CMV enhancer + chicken beta-actin promoter
标签/融合蛋白：· 8His-tag (N terminal on backbone) · mMBP with C-terminal TEV cleavage site linker (N terminal on backbone) · 6His-tag (C terminal on backbone) · Strep-tag II (C terminal on backbone) · HA-tag (C terminal on backbone)

克隆方法：限制性内切酶
5'测序引物：AAGGTGAAATCATGCCCAACATCC
3'测序引物：ATTTGTGAGCCAGGGCATTG

质粒图谱：