质粒名称：pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT
质粒编号：19291

载体名称：pGL2Basic
载体大小：5568
载体类型：Mammalian Expression, Luciferase

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：DH5alpha
高拷贝/低拷贝：高拷贝

插入基因：E-cadherin
物种来源：H. sapiens (human)
基因突变：E-cad promoter sequences from -108 to +125 Ebox elements were mutated from 5'-CANNTG-3' to 5'-AANNTA-3' (sense strand)
插入大小：233

克隆方法：限制性内切酶
5'克隆位点：SacI (not destroyed)
3'克隆位点：HindIII (not destroyed)
5'测序引物：unknown
3'测序引物：luc-rev

质粒图谱：