质粒名称:pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
质粒编号:117006
质粒备注:
Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
载体名称:pET30b+
载体类型:原核表达
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:低拷贝
插入基因:Fusicatenibacter saccharivorans RT-Cas1-Cas2
物种来源:Synthetic
启动子:T7
克隆方法:限制性内切酶
5'克隆位点:Unknown (unknown if destroyed)
3'克隆位点:Unknown (unknown if destroyed)
5'测序引物:T7
3'测序引物:T7-term
质粒图谱: