质粒名称:pET-Sac-Abeta(M1-42)
质粒编号:71875
质粒备注:
Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells
载体名称:pET vector
载体大小:4700
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:Abeta(M1-42)
基因别名:amyloid-beta 42
基因别名:abeta 42
物种来源:H. sapiens (human)
插入大小:129
基因名称:APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
启动子:T7
克隆方法:限制性内切酶
5'克隆位点:Nde1 (not destroyed)
3'克隆位点:Sac1 (not destroyed)
5'测序引物:5'- ATG GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG GTG -3'
3'测序引物:5'- GTG GGT TCT AAC AAG GGT GCT ATC ATC GGT CTG ATG GTT GGT GGC GTT GTG -3'
质粒图谱: