质粒名称:pET16B.Pfu
质粒编号:12509
质粒备注:
Bacterial expression of Pfu DNA polymerase
载体名称:pET16b
载体大小:5711
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Pfu DNA polymerase
物种来源:P. furiosus
基因突变:Y218H, Q462R, F533L, K559I and V683A (mutations were not intentional, but plasmid functions as described)
插入大小:2325
克隆方法:限制性内切酶
5'克隆位点:NdeI (not destroyed)
3'克隆位点:XhoI (not destroyed)
5'测序引物:TAATACGACTCACTATAGGG
3'测序引物:GCTAGTTATTGCTCAGCGG
质粒图谱: