质粒名称:pCS6-StaPLd-Casp9
质粒编号:111511
质粒备注:
Expresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
载体名称:pCMV-SPORT6
完整质粒大小:6801
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:StaPLd-Casp9
物种来源:H. sapiens (human); Hepatitis C Virus (HCV) genotype 1a
基因突变:The HCV NS3 protease carries V36M, T54A, and S122G mutations. Both caspase-9 copies have had their N termini truncated.
插入大小:2457
启动子:CMV
克隆方法:Gibson Cloning
5'测序引物:ACAGCTATGACCATTAGGCCT
3'测序引物:GTTGTAAAACGACGGCCAGT
质粒图谱: