质粒名称:pCRISPRyl
质粒编号:70007
质粒备注:
CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
载体名称:pUC19
载体大小:2623
完整质粒大小:11804
载体类型:Yeast Expression, CRISPR, Synthetic Biology
筛选标记:LEU2
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Codon optimized Cas9 from S. pyogenes
物种来源:Synthetic
插入大小:4140
启动子:UAS1B8-TEF(136)
克隆方法:限制性内切酶
5'克隆位点:BssHII (not destroyed)
3'克隆位点:NheI (not destroyed)
5'测序引物:GTAAAACGACGGCCAGT
3'测序引物:CAGGAAACAGCTATGAC
质粒图谱: