质粒名称:pCMV-PE2-P2A-GFP
质粒编号:132776
质粒备注:
Prime editing in mammalian cells with co-translational GFP expression
载体名称:pCMV
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:PE2-P2A-GFP
基因别名:Cas9(H840A)-M-MLVrt(D200N, T306K, W313F, T330P, L603W)-P2A-GFP
物种来源:Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
基因突变:See manuscript
插入大小:7170
启动子:CMV
标签/融合蛋白:· SV40 NLS (N terminal on backbone) · SV40 NLS (C terminal on backbone)
克隆方法:Ligation Independent Cloning
5'测序引物:T7-forward
3'测序引物:BGH-reverse
质粒图谱: