质粒名称:pCMV(MinDis).iGluSnFR
质粒编号:41732
质粒备注:
Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at **
载体名称:pCMV(MinDis) [variant of pDisplay]
载体大小:5300
完整质粒大小:6829
载体类型:Mammalian Expression ; Glutamate Biosensor
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:GltI based Glutamate Biosensor
基因别名:iGluSnFR
基因别名:gltI/ybeJ
基因别名:cpGFP146
物种来源:Synthetic; E. coli
基因突变:cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET inserted between AA253-254 of E. coli gltI/ybeJ
插入大小:1570
启动子:CMV
标签/融合蛋白:· mouse Ig κ-chain (N terminal on backbone) · Myc (C terminal on backbone) · PDGFR transmembrane helix (aa 513-561) (C terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:PstI (not destroyed)
5'测序引物:CMV-F
3'测序引物:BGH-rev
质粒图谱: