质粒名称:pCAG-T7-TALEN(Sangamo)-Destination
质粒编号:37184
质粒备注:
(Empty Backbone)Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
载体名称:pDIRECT
载体大小:4810
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
启动子:CAG
克隆方法:限制性内切酶
5'测序引物:TAL_F1 (ttggcgtcggcaaacagtgg)
3'测序引物:TAL_R2 (ggcgacgaggtggtcgttg
质粒图谱: