质粒名称:pAC149-pCR8-dCas9VP160
质粒编号:48221
质粒备注:
dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
载体名称:pCR8/GW/TOPO
载体大小:2799
完整质粒大小:7442
载体类型:CRISPR ; Gateway Donor
原核抗性:Spectinomycin, 50 μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:dCas9(D10A;H840A) fusion with VP160 activation domain
基因别名:dCas9VP160
物种来源:H. sapiens (human), Synthetic
基因突变:D10A;H840A nuclease-deficient
插入大小:4643
标签/融合蛋白:· HA Tag (N terminal on insert) · VP160 (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:M13F
3'测序引物:M13R
质粒图谱: