质粒名称:lenti-EF1a-dCas9-VPR-Puro
质粒编号:99373
质粒备注:
3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)
载体名称:pLenti
载体大小:9192
完整质粒大小:15702
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:Puromycin, Zeocin
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:(Sp)dCas9-VPR-P2A-Puro
物种来源:H. sapiens (human), M. musculus (mouse), Synthetic; ; S. pyogenes
插入大小:6510
启动子:EF-1a
克隆方法:Gibson Cloning
5'测序引物:GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
3'测序引物:cacatagcgtaaaaggagcaacatag
质粒图谱: