质粒名称:Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
质粒编号:118156
质粒备注:
Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
载体名称:lentiCRISPRv2
载体类型:哺乳动物表达,慢病毒,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:KRAB-dCas9-P2A-EGFP
物种来源:H. sapiens (human)
插入大小:4907
启动子:EF1a core and U6
克隆方法:Gibson Cloning
5'测序引物:TAAGAAGTACGGCGGCTTCG
质粒图谱: