质粒名称:lenti dCAS-VP64_Blast
质粒编号:61425
质粒备注:
3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
载体名称:plenti
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:Blasticidin
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:dCAS9(D10A, N863A)-VP64_2A_Blast
物种来源:Synthetic; S. pyogenes
基因突变:D10A and N863A in Cas9
启动子:EF1A
克隆方法:限制性内切酶
5'克隆位点:BsiWI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
3'测序引物:cacatagcgtaaaaggagcaacatag
质粒图谱: