质粒名称:IgK-IFN-luc
质粒编号:14886
质粒备注:
Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
载体名称:na
载体大小:5800
载体类型:Mammalian Expression, Luciferase
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:IgK-IFN
基因别名:IFNB1
基因别名:IgK2‐IFN‐LUC reporter
物种来源:H. sapiens (human)
插入大小:80
基因名称:IFNB1 (a.k.a. IFB, IFF, IFN-beta, IFNB)
克隆方法:限制性内切酶
5'克隆位点:SacII (not destroyed)
3'克隆位点:HindIII (not destroyed)
5'测序引物:na
3'测序引物:LucNrev
质粒图谱: