质粒名称:FUW-tetO-EGFP
质粒编号:84041
质粒备注:
3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
载体名称:FUW
载体大小:8482
完整质粒大小:10463
载体类型:哺乳动物表达,慢病毒
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:Enhanced Green Fluorescence Protein, Puromycin resistance gene
基因别名:EGFP-IRES-Puro
插入大小:1947
启动子:TRE-CMV
克隆方法:限制性内切酶
5'克隆位点:EcoRI (destroyed during cloning)
3'克隆位点:EcoRI (destroyed during cloning)
5'测序引物:LNCX
3'测序引物:pBluescriptKS
质粒图谱: