质粒名称:FUW mCherry-GFP-LC3
质粒编号:110060
质粒备注:
To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)
载体名称:FUW
载体类型:慢病毒
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:未知
插入基因:mCherry-GFP-LC3
克隆方法:TOPO Cloning
5'测序引物:CGCGGATCCGGTCGCCACCATGGTGAGCAAGGGCGAG
3'测序引物:CGCGGCGCGCCGCTGGGTCTAGATGCATGC
质粒图谱: