质粒名称:CROPseq-Guide-Puro
质粒编号:86708
质粒备注:
Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
载体名称:lentiGuide-Puro
完整质粒大小:10214
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:EF1a-Puro-WPRE-hU6-gRNA
物种来源:Synthetic
启动子:hU6
克隆方法:Gibson Cloning
5'测序引物:TTGGGCACTGACAATTCCGT
质粒图谱: