质粒名称:c-Flag pcDNA3
质粒编号:20011
质粒备注:
(Empty Backbone)This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
载体名称:pcDNA3.1
载体大小:5400
载体类型:哺乳动物表达
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:None
克隆方法:限制性内切酶
5'克隆位点:HindIII, XbaI, XhoI (not destroyed)
3'克隆位点:KpnI, BamHI (not destroyed)
5'测序引物:T7
3'测序引物:SP6
质粒图谱: