质粒名称:AAV pEF1a-DIO-FLPo-WPRE-hGHpA
质粒编号:87306
质粒备注:
An AAV vector expresses FLPo in a Cre recombinase-dependent manner.
载体名称:pAAV
载体大小:5601
完整质粒大小:6892
载体类型:Mammalian Expression, AAV
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:Cre-dependent mouse codon-optimized Flp recombinase.
基因别名:DIO-FLPo
物种来源:M. musculus (mouse), S. cerevisiae (budding yeast), Synthetic
插入大小:1299
启动子:EF1a
克隆方法:限制性内切酶
5'克隆位点:NheI (not destroyed)
3'克隆位点:AscI (not destroyed)
5'测序引物:AGCTGACAGGTGGTGGCAAT
3'测序引物:TCAAGCCTCAGACAGTGGTTC
质粒图谱: