质粒名称:6-His-MBP-TEV-FnCpf1
质粒编号:90094
质粒备注:
Bacterial expression plasmid for protein purification
载体名称:pET-28
载体大小:6643
完整质粒大小:10553
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:FnCpf1 (humanized)
基因别名:type V CRISPR-associated protein Cpf1 (humanized)
基因别名:Cpf1 (humanized)
基因别名:Francisella tularensis subsp. novicida U112 Cpf1 (humanized)
物种来源:Francisella tularensis subsp. novicida U112
插入大小:3910
启动子:T7
标签/融合蛋白:· 6xHis (N terminal on backbone) · MBP (N terminal on insert) · TEV site (N terminal on insert) · Nucleoplasmin NLS (C terminal on insert) · 3xHA (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:NcoI (unknown if destroyed)
3'克隆位点:XhoI (not destroyed)
5'测序引物:taatacgactcactataggg?
质粒图谱: