蛋白质印迹:

Western blot analysis of recombinant IDH2(R172W) and wildtype proteins.

Purified His-tagged IDH2(R172W) (lane 2) and wildtype protein (lane 1) were blotted with anti IDH2(R172W) monoclonal antibody (Cat. #26164).

免疫荧光:

Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R172W) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172W) plasmid (right column), then fixed and stained with anti-IDH2(R172W) monoclonal antibody (Cat. #26164).