SED52100 SequiTherm EXCEL? II DNA Polymerase

半岛bd体育手机客户端 简介：北京中北林格供应SED52100 SequiTherm EXCEL? II DNA Polymerase，中北林格是lucigen总代理，提供lucigen半岛bd体育手机客户端 货号。其他包括cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。

半岛bd体育手机客户端 名称:

SequiTherm EXCEL? II DNA Polymerase

货号:SED52100

规格:100 Rxns

存储温度:-20℃

品牌：lucigen / epicentre

供应商：中北林格

产地：us

发货地：北京

SED52100 SequiTherm EXCEL? II DNA Polymerase 半岛bd体育手机客户端 描述

• Automated sequencing of all DNA templates, including those containing difficult-to-sequence regions that cannot be resolved with other sequencing kits (Figs. 1-4).

• 对所有DNA模板进行自动测序，包括那些含有难以用其他测序试剂盒解析的难以测序区域的DNA模板（图1-4）。

SequiTherm EXCEL II? Sequencing Kits for automated sequencers use the SequiTherm EXCEL II Polymerase, which improves sequencing performance with increased signal intensities and the ability to sequence through GC-rich regions. The SequiTherm EXCEL II DNA Sequencing Kits incorporate the use of betaine,* which enables sequencing of a broad range of templates, including those containing regions that are very difficult to sequence. Protocols are provided for both cycle and high-temperature isothermal (noncycle) sequencing.

用于自动测序仪的SequiTherm EXCEL II?测序试剂盒使用SequiTherm EXCEL II聚合酶，该酶可提高测序性能，同时增加信号强度，并能够通过富含GC的区域进行测序。SequiTherm EXCEL II DNA测序试剂盒结合了甜菜碱的使用，*可以对多种模板进行测序，包括那些很难测序的区域。提供了用于循环和高温等温（非循环）测序的方案。

The kits have been optimized for use with the following DNA sequencers: LI-COR? 4000 and 4200 series Sequencers, NEN? Global IR²DNA Sequencers, ALF? DNA Sequencers, and ABI PRISM? DNA Sequencers using dye primer technology. Using primers labeled with standard sequencing dyes, the SequiTherm EXCEL II kits may also be used with ABI PRISM 373 and 377 series sequencers. The kits are not compatible with ABI capillary sequencers (ABI 310, 3100, or 3700 series) or with BigDye? technology.

该试剂盒经过优化，可与以下DNA测序仪配合使用：LI-COR?4000和4200系列测序仪，NEN?Global IR²DNA测序仪，ALF?DNA测序仪和使用染料引物技术的ABI PRISM?DNA测序仪。使用标有标准测序染料的引物，SequiTherm EXCEL II试剂盒还可与ABI PRISM 373和377系列测序仪一起使用。该套件与ABI毛细管测序仪（ABI 310、3100或3700系列）或BigDye?技术不兼容。

Two different kits are available for use with LI-COR? and NEN? sequencing equipment. The first kit (Cat. No. SE9101LC) contains Termination Mixes that give optimal read lengths up to about 1,000 bases and is designed for use on 25- to 41-cm gels. The second kit (Cat. No. SE9202LC) provides enhanced signal intensities in the resolution range of 1,000-1,400 bases and is ideal for users interested in obtaining the longest possible reads in conjunction with 66-cm gels, version 4.x of the BaseImagIR? software, and all versions of e-Seq? software.

有两种不同的套件可与LI-COR?和NEN?测序设备一起使用。第×个试剂盒（目录号SE9101LC）包含终止混合物，可提供长达1000个碱基的最佳读取长度，并设计用于25至41厘米的凝胶。第二个试剂盒（目录号SE9202LC）在1,000-1,400碱基的分辨率范围内提供增强的信号强度，对于希望与66-cm凝胶（版本4.x的BaseImagIR）一起获得尽可能长的读数的用户来说是理想的选择?软件以及所有版本的e-Seq?软件。

好处

• 序列模板包含发夹结构（图3），高GC或AT区域（图1），链间重退火区域或核苷酸重复序列（图4）。

• 降低的背景信号可产生更清晰，更少歧义的序列数据（图2）。

• 与LI-COR，NEN和ALF DNA测序仪（染料引物和内部标签）以及ABI PRISM自动测序仪（染料引物）兼容的试剂盒。

• 可选的等温方案，可对循环测序不利的模板进行测序。终止混合物包含7-脱氮-dGTP，以减少凝胶电泳过程中的条带压缩。

• Sequence templates containing hairpin structures (Fig. 3), regions of high GC or AT (Fig. 1) content, areas of interstrand reannealing, or nucleotide repeats (Fig. 4).

• Reduced background signal results in clearer, less ambiguous sequence data (Fig. 2).

• Kits compatible with LI-COR, NEN, and ALF DNA sequencers (both dye-primer and internal label) and with ABI PRISM automated sequencers (dye-primer).

• Optional isothermal protocol to sequence templates that are recalcitrant to cycle sequencing. Termination Mixes contain 7-deaza-dGTP to reduce band compression during gel electrophoresis.

SED52100 SequiTherm EXCEL? II DNA Polymerase 图例说明：

图1.困难模板的排序。 使用标准循环测序试剂盒（A）和SequiTherm EXCEL?II试剂盒LC（B）对富含AT的模板进行测序。如图所示，使用SequiTherm EXCEL II Kit-LC消除了几个牢固的限位，并易于读取序列。样品以G，A，T，C顺序加载。数据在4000型DNA测序仪上可视化。

图2. SequiTherm EXCEL?II Kit-LC大大降低了非特异性背景。 用标准循环测序试剂盒（A）和SequiTherm EXCEL II试剂盒LC（B）对质粒DNA模板进行测序。使用SequiTherm EXCEL II Kit-LC可以大大降低背景信号。样品以G，A，T，C顺序加载。数据在4000型DNA测序仪上可视化。

图3.当其他方法失败时，SequiTherm EXCEL II?DNA测序试剂盒可提供完整，清晰的测序数据。 使用标准循环测序方案和IRD800标记的引物，使用SequiTherm EXCEL II DNA测序试剂盒-LC成功地测序了一个包含推定的24 bp发夹环的超螺旋质粒。数据在IR2型4000L DNA测序仪（面板A）上可视化。使用ABI BigDye?终止子循环测序试剂盒无法解析相同的区域。数据在ABIPRISM?310遗传分析仪（面板B）上可视化。

图4.通过有问题的重复区域进行测序。 使用SequiTherm EXCEL?II DNA测序试剂盒，使用标准循环测序方案和染料标记的引物，成功地对包含GA-二核苷酸重复序列（A），GGC三核苷酸重复序列（B）或GAAA-四核苷酸重复序列（C）的超螺旋质粒进行了测序。

SED52100 SequiTherm EXCEL? II DNA Polymerase 货号详情
Catalog No.Size

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SequiTherm EXCEL? II DNA Sequencing Kit - LC for 25-41 cm gelsSE9101LC100 SequencesFor LI-COR ^?or NEN ^?Global IR ²Sequencers.

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SequiTherm EXCEL? II DNA Sequencing Kit - LC for 66 cm gelsSE9202LC100 SequencesFor LI-COR ^?or NEN ^?Global IR ²Sequencers. Contents:SequiTherm EXCEL? II DNA Polymerase, 3.5X SequiTherm EXCEL? II Sequencing Buffer, SequiTherm EXCEL? II-LC Termination Mixes (G, A, T, C), Control Template, Stop/Loading Buffer, Sterile Deionized Water.

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SequiTherm EXCEL? II DNA PolymeraseSED52100100 ReactionsIncludes SequiTherm EXCEL? II Sequencing Buffer.

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Stop/Gel Loading Buffer, Basic Fuchsin and Bromophenol Blue DyesSF810151.5 mlFor LI-COR ^?, NEN ^?. Sufficient for 100 sequences.

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Basic Fuchsin DyeSA850252.5 mlFor ALF ^?, ABI PRISM ^?, LI-COR ^?, NEN ^?. Sufficient for 100 sequences.
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