您好，欢迎来到半岛电竞官方网址 ！ [ 登录] [ 免费注册]
半岛电竞官方网址
发布求购
位置： 首页> 半岛bd体育手机客户端 库> Licochalcone D
立即咨询
咨询类型：
*姓名：
*电话：
*单位：
Email：
*留言内容：
请详细说明您的需求。
*验证码：
Licochalcone D
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Licochalcone D图片
CAS NO: 144506-15-0
包装与价格：
包装 价格(元)
10 mM * 1 mL in DMSO 电议
1mg 电议
5mg 电议
10mg 电议
50mg 电议

半岛bd体育手机客户端 名称
甘草查尔酮 D
半岛bd体育手机客户端 介绍
Licochalcone D 是存在于 Glycyrrhiza uralensis中的黄酮类化合物，是具有口服活性的 NF-κB p65的有效抑制剂。Licochalcone D 具有抗氧化、抗炎、抗癌等作用。
生物活性

Licochalcone D, a flavonoid compound mainly existing in the root ofGlycyrrhiza uralensis, is a potent and orally active inhibitor ofNF-kappaB (NF-κB) p65. Licochalcone D possesses antioxidant, anti-inflammatory, anti-cancer properties[1][2].
IC50& Target[1]

p65
体外研究
(In Vitro)

Licochalcone D (10 μM; 1 h) negatively regulatesLipopolysaccharides(LPS) (HY-D1056)-induced NF-κB activation by inhibiting the phosphorylation and transactivation of NF-κB p65, inhibits the LPS-induced activation of protein kinase A (PKA), and significantly inhibits the LPS-induced expression of TNFα and MCP-1[1].
Licochalcone D (10 μM; 24 h) significantly inhibits LPS-induced iNOS expression and NO production[1].
Licochalcone D (0-90 μM; 24 h) inhibits the proliferation of human melanoma cells, induces the apoptosis of A375 cells, decreases the mitochondrial membrane potential and increases ROS production in A375 cells[2].
Licochalcone D (0-25 μM; 24 h) decreases the migration and invasion ability of the A375 cells, decreased the protein levels of MMP-2 and MMP-9[2].

Western Blot Analysis[1]

Cell Line: RAW264.7 cells
Concentration: 10 μM
Incubation Time: 1 h or 24 h (iNOS), following LPS stimulation (1 μg/mL) for 30 min
Result: Inhibited the phosphorylation of NF-κB at serine 276 but not at serine 536.
Inhibited the LPS-induced expression of TNFα mRNA and MCP-1 mRNA.
Strongly inhibited the expression of iNOS.

Cell Viability Assay[2]

Cell Line: A375 cells or SK-MEL-5
Concentration: 0, 1, 2.5, 5, 15, 30, 45, 60, 75 and 90 μM (A375) or 0, 20, 40, 60 and 80 μM (SK-MEL-5)
Incubation Time: 24 h
Result: Inhibited the proliferation of A375 and SK-MEL-5 in a concentration dependent manner.

Apoptosis Analysis[2]

Cell Line: A375
Concentration: 0, 30, 60 and 90 μM
Incubation Time: 24 h
Result: Cells exhibited obvious apoptotic characteristics after the treatment.

RT-PCR[2]

Cell Line: A375
Concentration: 0, 30, 60 and 90 μM
Incubation Time: 24 h
Result: Downregulated the mRNA level of Bcl-2 and upregulated the mRNA levels of caspase-3, caspase-9 and Bax.

Cell Invasion Assay[2]

Cell Line: A375
Concentration: 25 μM
Incubation Time: 24 h
Result: Significantly suppressed cell invasion.

Cell Migration Assay[2]

Cell Line: A375
Concentration: 25 μM
Incubation Time: 24 h
Result: Significantly suppressed cell migration in the A375 cells.
体内研究
(In Vivo)

Licochalcone D (25 and 50 mg/kg; i.g.; daily for a week) inhibits the tumor growth in a mouse xenograft model of murine melanoma B16F0 cells[2].

Animal Model: C57BL/6 mice, B16F0 tumor model[2]
Dosage: 25 and 50 mg/kg
Administration: Intragastric administration, daily for a week
Result: Obviously lowered the tumor growth rates, the tumor growth inhibition rates were calculated to be 32.0 and 54.1% at 25 and 50 mg/kg, respectively.
分子量

354.40
性状

Solid
Formula

C21H22O5
CAS 号

144506-15-0
中文名称

甘草查尔酮 D
结构分类
  • Flavonoids
  • Chalcones
  • Phenols
  • Polyphenols
来源
  • Plants
  • Leguminosae
  • Glycyrrhiza uralensisFisch.
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro:

DMSO : 125 mg/mL(352.71 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 2.8217 mL 14.1084 mL 28.2167 mL
5 mM 0.5643 mL 2.8217 mL 5.6433 mL
10 mM 0.2822 mL 1.4108 mL 2.8217 mL
*

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO40%PEG3005%Tween-8045% saline

    Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.87 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂： 10% DMSO90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.87 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
* 以上所有助溶剂都可在本网站选购。
Baidu
map