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Glycycoumarin
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Glycycoumarin图片
CAS NO: 94805-82-0
包装与价格：
包装 价格(元)
10 mM * 1 mL in DMSO 电议
5mg 电议
10mg 电议
50mg 电议
100mg 电议

半岛bd体育手机客户端 名称
甘草香豆素
半岛bd体育手机客户端 介绍
Glycycoumarin 是一种有效的抗痉挛剂。Glycycoumarin 是甘草的主要生物活性香豆素，具有抗痉挛活性。Glycycoumarin 还具有保肝作用。Glycycoumarin 可用于腹痛和肝脏疾病的研究。
生物活性

Glycycoumarin is a potentantispasmodicagent. Glycycoumarin is a major bioactive coumarin of licorice and exhibits antispasmodic activity. Glycycoumarin also has hepatoprotective effect. Glycycoumarin can be used for the research of abdominal pain and liver diseases[1][2].
体外研究
(In Vitro)

Glycycoumarin, (25 μM; 24 h) combining with ABT-737 (12.5 μM) synergistically, induces cell death in multiple types of liver cancer cell HepG2[2].
Glycycoumarin is highly effective against alcoholic liver disease, nonalcoholic fatty liver disease, acetaminophen-induced hepatotoxicity, and liver cancer through mechanisms involved in activation of Nrf2 antioxidant system, stimulation of AMPK-mediated energy homeostasis, induction of autophagy degradation process, and inhibiting oncogenic kinase T-lymphokine-activated killer cell-originated protein kinase activity[3].

Cell Viability Assay[2]

Cell Line: HepG2, SMMC-7721, and Huh-7
Concentration: 10, 20, 25, 30, and 40 μM
Incubation Time: 24 hours
Result: Induced cell death in multiple types of liver cancer cell lines in a dose-dependent manner.
体内研究
(In Vivo)

Glycycoumarin (30 μM-0.3 nM; 5 min) has an inhibitory effect on smooth muscle contraction induced by various types of stimulants through the inhibition of PDEs, especially isozyme 3, followed by the accumulation of intracellular cAMP[1]. Glycycoumarin (10 mg/kg; i.p.; once daily for 4 d) enhances tumor growth inhibition in HepG2 xenograft model in mice and shows synergistical effect withABT-737(HY-50907)[2].

Animal Model: Male ICR mice (aged 6 weeks, weight 25-30 g)[1]
Dosage: 30 μM-0.3 nM, 5 min
Administration:
Result: Inhibited the contraction induced by various types of stimulants, such as CCh, KCI, BaCI(2), and A23187 (calcium ionophore III).
Enhanced the relaxation induced by forskolin on CCh-evoked contraction and also enhances the relaxation effect of rolipram.
Associated with dose-dependent accumulation of cAMP.
Animal Model: HepG2 cancer cells xenograft model in male BALB/c athymic nude mice (6-7 weeks old)[2]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; once daily for 4 days; ABT-737 group was given 100 mg/kg i.p.
Result: Inhibited tumor growth and resulted a reduction of the final tumor weight by 17%.
分子量

368.38
性状

Solid
Formula

C21H20O6
CAS 号

94805-82-0
中文名称

甘草香豆素
结构分类
  • Phenylpropanoids
  • Coumarins
  • Phenols
  • Polyphenols
来源
  • Plants
  • Leguminosae
  • Glycyrrhiza uralensisFisch.
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro:

DMSO : 250 mg/mL(678.65 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 2.7146 mL 13.5729 mL 27.1459 mL
5 mM 0.5429 mL 2.7146 mL 5.4292 mL
10 mM 0.2715 mL 1.3573 mL 2.7146 mL
*

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO40%PEG3005%Tween-8045% saline

    Solubility: ≥ 2.08 mg/mL (5.65 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.65 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂： 10% DMSO90%corn oil

    Solubility: 2.08 mg/mL (5.65 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (5.65 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

* 以上所有助溶剂都可在本网站选购。
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