Lycopodine，一种石松 (
Lycopodium clavatumspores) 孢子重要的生物活性成分，通过调节难治性前列腺癌细胞中
5-脂氧合酶 (5-lipoxygenase)，极化线粒体膜电位而不调节 p53 活性来诱导细胞凋亡。 Lycopodine 通过激活
caspase-3诱导细胞凋亡来抑制 HeLa 细胞的增殖。
| 生物活性 |
Lycopodine, a pharmacologically important bioactive component derived fromLycopodium clavatumspores, triggersapoptosisby modulating5-lipoxygenase, and depolarizing mitochondrial membrane potential in refractory prostatecancercells without modulating p53 activity[1]. Lycopodine inhibits proliferation of HeLa cells through induction ofapoptosisviacaspase-3activation[2].
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| IC50& Target[2] |
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体外研究
(In Vitro) |
Lycopodine (5.22-78.3 μg/mL; 12 hours) has 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively[1].
Treated with Lycopodine (74-222 mM; 12 hours), the apoptotic index is with respect to the gradual increase in doses for the PC3 and LnCaP cells[1].
Lycopodine (74-222 mM; 12 hours) induces cell cycle arrest at G0/G1 phase in PC3 and LnCaP cells[1].
Lycopodine (0-200 μg/mL; 48 hours) shows cytotoxicity to HeLa cells in a dose and time dependent manner. However, Lycopodine shows minimal cytotoxic effects in normal peripheral blood mononuclear cells (PBMC) even at the highest dose (200 μg/mL)[2].
Lycopodine (100, 200 μg/mL; 24 hours) increases level of Bax and decreases the mitochondrial cytochrome c. This is followed by an increase in expression of cytochrome c in cytosolic fraction. Lycopodine also cleaves the caspase-3 in the total cell lysate, while the expression of Bcl-2 is down regulated[2].
Cell Viability Assay[1]
| Cell Line: |
PC3 and LnCaP cells |
| Concentration: |
5.22-78.3 μg/mL |
| Incubation Time: |
12 hours |
| Result: |
Reached 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively. |
Apoptosis Analysis[1]
| Cell Line: |
PC3 and LnCaP cells |
| Concentration: |
74, 148, 222 mM |
| Incubation Time: |
12 hours |
| Result: |
The apoptotic index was with respect to the gradual increase in doses. |
Cell Cycle Analysis[1]
| Cell Line: |
PC3 and LnCaP cells |
| Concentration: |
74, 148, 222 mM |
| Incubation Time: |
12 hours |
| Result: |
Arrested growth of the cells at G0/G1 phase in the case of PC3 and LnCaP cells. |
Cell Cytotoxicity Assay[2]
| Cell Line: |
Hela cells and PBMC |
| Concentration: |
0-200 μg/mL |
| Incubation Time: |
48 hours |
| Result: |
A linear increase of the cytotoxicity was along with the increase of time of treatment and also of the dose. |
Western Blot Analysis[2]
| Cell Line: |
HeLa cells |
| Concentration: |
100, 200 μg/mL |
| Incubation Time: |
24 hours |
| Result: |
Increased level of Bax and decreased the mitochondrial cytochrome c. |
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| 分子量 |
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| Formula |
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| CAS 号 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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