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28-Deoxonimbolide
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
28-Deoxonimbolide图片
CAS NO: 126005-94-5
包装与价格：
包装 价格(元)
100mg 电议
250mg 电议
500mg 电议

半岛bd体育手机客户端 介绍
28-Deoxonimbolide 是一种宁宾 (nimbin, HY-N3187) 型柠檬苦素，可以从 Azadirachta indica种子提取物中分离出来。28-Deoxonimbolide 具有抗癌活性。 28-Deoxonimbolide 通过线粒体和死亡受体介导的途径诱导 HL60 细胞凋亡 ( apoptosis)。
生物活性

28-Deoxonimbolide is animbin(HY-N3187) type limonoid, that can be isolated fromAzadirachta indicaseed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide inducesapoptoticcell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways[1].
IC50& Target

Caspase-8

Caspase-9

Caspase-3
体外研究
(In Vitro)

28-Deoxonimbolide (1-100 μM，48 小时) 对 HL60 (白血病)、A549 (肺)、AZ521 (胃)、SK-BR-3 (乳腺)、CRL1579 (黑色素瘤)和 RPMI1788 (正常淋巴细胞) 显示强有力的细胞毒性[1]。
28-Deoxonimbolide (30 μM, 0-24 h) 诱导 HL60 细胞凋亡[1]。
28-Deoxonimbolide (30 μM, 0-24 h) 可增加裂解的 caspases-8、-9 和 -3 的水平[1]。

Cell Cytotoxicity Assay[1]

Cell Line: HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788
Concentration: 1-100 μM
Incubation Time: 48 h
Result: Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively.

Apoptosis Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control).

Western Blot Analysis[1]

Cell Line: HL60 cells
Concentration: 30 μM
Incubation Time: 8 and 24 h
Result: Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3.
分子量

452.54
Formula

C27H32O6
CAS 号

126005-94-5
结构分类
  • Terpenoids
  • Triterpenes
来源
  • Plants
  • Meliaceae
  • Azadirachta indicaA. Juss.
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
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