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Lobaplatin
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Lobaplatin图片
CAS NO: 135558-11-1
包装与价格：
包装 价格(元)
10 mM * 1 mL in Water 电议
1mg 电议
5mg 电议
10mg 电议
50mg 电议
100mg 电议
200mg 电议

半岛bd体育手机客户端 名称
络铂
D-19466
半岛bd体育手机客户端 介绍
Lobaplatin (D-19466) 是铂 (II) 络合物的非对映混合物。Lobaplatin 将细胞周期阻滞在 G1 和 G2/M 期。Lobaplatin 通过增加 caspase和 Bax 的表达，降低 Bcl-2 的表达来诱导细胞凋亡 ( apoptosis)。Lobaplatin 可用于癌症研究。
生物活性

Lobaplatin (D-19466) is a diastereometric mixture of platinum(II) complexe. Lobaplatin arrests cell cycle at G1 and G2/M phase. Lobaplatin inducesapoptosisby increasing expressions ofcaspaseandBax, decreasing expression ofBcl-2. Lobaplatin can be used for research ofcancer[1][2][3].
体外研究
(In Vitro)

Lobaplatin (D-19466, 0.25-32 μg/mL; 24-72 h) exhibits anti-proliferative activities against esophageal squamous cell carcinoma (ESCC) cell lines[1].
Lobaplatin (0-16 μg/mL; 48 h) induces esophageal squamous cell carcinoma (ESCC) apoptosis and modulates expression of apoptosis-related proteins[1].
Lobaplatin (1.45 μg/mL; 0-48 h; SMMC-7721 cells) arrests cell cycle progression at G1 and G2/M phases in a time-dependent manner[2].
Lobaplatin (1.45 μg/mL; 0-48 h; SMMC-7721 cells) inhibits the mRNA levels of cyclin B, CDK1, and CDC25C phosphatase, down-regulates Rb/E2F complexes and up-regulates of CDK inhibitors[2].

Cell Viability Assay[1]

Cell Line: KYSE-410 cells and EC-109 cells
Concentration: 0.25, 0.5, 1, 2, 4, 8, 16 and 32 μg/mL
Incubation Time: 24, 48 and 72 hours
Result: Inhibited the growth of KYSE-410 and EC-109 cells in a dose- and time-dependent manner.
Inhibited the clone formation activity of KYSE-410 and EC-109 cells in a dosedependent manner.

Apoptosis Analysis[1]

Cell Line: KYSE-410 cells and EC-109 cells
Concentration: 0.25, 1, 4 and 16 μg/mL
Incubation Time: 48 hours
Result: Increased the percentage of apoptotic cells in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: KYSE-410 cells and EC-109 cells
Concentration: 0, 1, 4 and 16 μg/mL
Incubation Time: 48 hours
Result: Increased expressions of cleaved-caspase-3, cleaved-caspase-8, cleaved-caspase-9 and Bax, while decreased expression of Bcl-2.

Cell Cycle Analysis[2]

Cell Line: SMMC-7721 cells
Concentration: 1.45 μg/mL
Incubation Time: 0, 24, 36 and 48 hours
Result: Arrested the proportions of G1, S, and G2/M phases in cells were 45.31, 22.88, and 31.81% at 0 h, 59.91, 11.92, and 28.17% at 24 h, 56.89, 2.83, and 40.28% at 36 h, and 53.80, 2.07, and 44.13% at 48 h, respectively.

Western Blot Analysis[2]

Cell Line: SMMC-7721 cells
Concentration: 1.45 μg/mL
Incubation Time: 0, 24, 36 and 48 hours
Result: Down-regulated cyclin B, CDK1, CDC25C, phosphorylated CDK1 (pCDK1), pCDK4, Rb, E2F, and pRb, and up-regulated p53, p21, and p27.
体内研究
(In Vivo)

Lobaplatin (5 and 10 mg/kg; i.p.; once a week, for 3 weeks) suppresses tumor growth of esophageal squamous cell carcinoma (ESCC) xenograft[1].

Animal Model: Male BALB/c nude mice (4-6 weeks) with ESCC xenograft[1]
Dosage: Intraperitoneal injection; once a week, for 3 weeks
Administration: 5 and 10 mg/kg
Result: Suppressed tumor volumes in a dose-dependent manner.
Increased expressions of Bax and decreased expressions of Bcl-2.
Clinical Trial
分子量

397.33
性状

Solid
Formula

C9H18N2O3Pt
CAS 号

135558-11-1
中文名称

络铂
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro:

H2O : ≥ 50 mg/mL(125.84 mM;DMSO can inactivate Lobaplatin's activity)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 2.5168 mL 12.5840 mL 25.1680 mL
5 mM 0.5034 mL 2.5168 mL 5.0336 mL
10 mM 0.2517 mL 1.2584 mL 2.5168 mL
*

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： PBS

    Solubility: 100 mg/mL (251.68 mM); Clear solution; Need ultrasonic

* 以上所有助溶剂都可在本网站选购。
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