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K-7174 dihydrochloride
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
K-7174 dihydrochloride图片
CAS NO: 191089-60-8
包装与价格：
包装 价格(元)
10 mM * 1 mL in Water 电议
5mg 电议
10mg 电议
50mg 电议
100mg 电议
200mg 电议

半岛bd体育手机客户端 介绍
K-7174 dihydrochloride 是具有口服活性的 proteasome和 GATA的抑制剂。K-7174 dihydrochloride 抑制 细胞黏附。K-7174 dihydrochloride 诱导细胞凋亡。K-7174 dihydrochloride 具有抗肿瘤活性，可用于癌症的研究。
生物活性

K-7174 dihydrochloride is an orally activeproteasomeandGATAinhibitor. K-7174 dihydrochloride is acell adhesioninhibitor. K-7174 dihydrochloride induces cellapoptosis. K-7174 dihydrochloride shows antitumor activities, it can be used for the research ofcancer[1][2][3].
体外研究
(In Vitro)

K-7174 dihydrochloride (10 μM; 1 h) inhibits the adhesion by VCAM-1 and its ligand[1].
K-7174 dihydrochloride (1-30 μM; 1 h) dose-dependently suppresses the VCAM-1 expression with an IC50value of 14 μM[1].
K-7174 dihydrochloride (1-30 μM; 1 h) dose-dependently suppresses the induction of VCAM-1 mRNA by TNFα with an IC50value of 9 μM[1].
K-7174 dihydrochloride (10-20 μM; 24 h) dose-dependently rescues Epo production by Hep3B cells[2].
K-7174 dihydrochloride (2.5-30 μM; 24 h) inhibits the binding activity of GATA[2].
K-7174 dihydrochloride (0-25 μM; 72 h) inhibits MM cells growth and induces cell apoptosis[3].

Cell Viability Assay[3]

Cell Line: KMS12-BM, U266, and RPMI8226 cell lines
Concentration: 0-25 μM
Incubation Time: 72 h
Result: Inhibited MM cells growth.

Apoptosis Analysis[3]

Cell Line: KMS12-BM, U266, and RPMI8226 cell lines
Concentration: 10 μM
Incubation Time: 48 h
Result: Significantly increased apoptosis of MM cells with the increasing percentage of annexin-V-positive cells.
体内研究
(In Vivo)

K-7174 dihydrochloride (30 mg/kg; i.p. once daily for 9 days) reverses the decreasing of hemoglobin concentrations and reticulocyte counts by IL-1β or TNF-α[2].
K-7174 dihydrochloride (75 mg/kg; i.p. once daily for 14 days) inhibits the tumor growth in vivo[3].
K-7174 dihydrochloride (50 mg/kg; p.o. once daily for 14 days) inhibits the tumor growth in vivo and shows a better effect than intraperitoneal injection[3].

Animal Model: ICR mice with IL-β or TNF-α injection[2]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection; 30 mg/kg once daily for 9 days
Result: Increased erythropoietin (Epo) production, reticulocyte counts, and hemoglobin (Hb) concentrations.
Animal Model: NOD/SCID mice with murine xenograft[3]
Dosage: 75 mg/kg
Administration: Intraperitoneal injection; once daily for 14 days
Result: Significantly decreased tumor volume, but showed a significant body weight reduction after 10 days.
Animal Model: NOD/SCID mice with murine xenograft[3]
Dosage: 50 mg/kg
Administration: Oral gavage; once daily for 14 days
Result: Showed an anti-myeloma activity. Porved oral administration is more effective than intraperitoneal injection.
分子量

641.67
性状

Solid
Formula

C33H50Cl2N2O6
CAS 号

191089-60-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:

H2O : 15 mg/mL(23.38 mM;Need ultrasonic and warming)

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 1.5584 mL 7.7922 mL 15.5843 mL
5 mM 0.3117 mL 1.5584 mL 3.1169 mL
10 mM 0.1558 mL 0.7792 mL 1.5584 mL
*

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： PBS

    Solubility: 10 mg/mL (15.58 mM); Clear solution; Need ultrasonic

* 以上所有助溶剂都可在本网站选购。
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